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Related Experiment Videos

Ubc9 interacts with SOX4 and represses its transcriptional activity.

Xin Pan1, Huiyan Li, Peijing Zhang

  • 1National Center of Biomedical Analysis, Beijing 100850, PR China.

Biochemical and Biophysical Research Communications
|April 25, 2006
PubMed
Summary

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Human ubiquitin-conjugating enzyme 9 (hUbc9) interacts with SOX4, a key transcription factor. hUbc9 represses SOX4 activity, independent of its SUMO-1 conjugation function, impacting cellular processes.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • SOX4 is a crucial transcription factor within the SOX family, regulating numerous cellular functions.
  • Understanding SOX4 regulation is vital for deciphering its role in various biological processes.

Purpose of the Study:

  • To identify proteins interacting with SOX4.
  • To elucidate the functional consequences of SOX4-interacting proteins on SOX4 transcriptional activity.

Main Methods:

  • Yeast two-hybrid screening to identify SOX4 interacting partners.
  • GST pull-down and co-immunoprecipitation assays to confirm interactions.
  • Confocal microscopy for subcellular localization studies.
  • Luciferase reporter assays to assess transcriptional activity.

Related Experiment Videos

Main Results:

  • Human ubiquitin-conjugating enzyme 9 (hUbc9) was identified as a SOX4 interacting protein.
  • The HMG-box domain of SOX4 mediates interaction with hUbc9.
  • hUbc9 co-localizes with SOX4 in the nucleus.
  • hUbc9 represses SOX4 transcriptional activity in both 293T and T47D cells.
  • This repression is independent of hUbc9's SUMO-1 conjugation activity.

Conclusions:

  • hUbc9 directly interacts with SOX4.
  • hUbc9 negatively regulates SOX4 transcriptional activity.
  • The interaction and repressive function of hUbc9 on SOX4 are independent of its SUMOylation activity, suggesting novel regulatory mechanisms.