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HPLC determination of phenolics adsorbed on yeasts.
M Rizzo1, D Ventrice, M A Varone
1Dipartimento di Scienze Farmacobiologiche, Facoltà di Farmacia, Università Magna Graecia di Catanzaro, Viale Europa, Località Germaneto, 88100 Catanzaro, Italy. rizzomilena@unicz.it
Journal of Pharmaceutical and Biomedical Analysis
|April 25, 2006
Summary
A new HPLC method accurately quantifies phenolic compounds like catechin (CA), rutin (RU), and gallic acid (GA) adsorbed onto yeast cell walls. This method aids in understanding yeast interactions with these important grape-derived compounds.
Area of Science:
- Analytical Chemistry
- Microbiology
- Food Science
Background:
- Phenolic compounds from grape byproducts are increasingly utilized in food and beverage industries.
- Understanding the adsorption of these phenolics onto yeast cell walls, such as Saccharomyces cerevisiae, is crucial for optimizing industrial processes.
- Key phenolics include monomeric anthocyanins (like catechin), flavonols (like rutin), and phenol acids (like gallic acid).
Purpose of the Study:
- To develop and validate a robust analytical High-Performance Liquid Chromatography (HPLC) method.
- To quantify specific phenolic compounds adsorbed onto the cell walls of Saccharomyces cerevisiae strains.
- To assess the adsorption profiles of gallic acid, catechin, and rutin on yeast grown in media rich in grape-derived phenolics.
Main Methods:
- Development of an analytical HPLC method utilizing reversed-phase chromatography.
- Sample preparation involving liquid-liquid extraction for microbial biomass purification.
- Detection of gallic acid (GA), catechin (CA), and rutin (RU) using an ultraviolet detector.
Main Results:
- The HPLC method demonstrated linearity over the tested concentration ranges for GA, CA, and RU (R² > 0.9983).
- High recovery rates were achieved: >85% for GA and RU, and >94% for CA.
- Low detection limits were established: 0.015 µg/mg for GA, 0.025 µg/mg for CA, and 0.029 µg/mg for RU.
Conclusions:
- The developed HPLC method is highly responsive and accurate for determining adsorbed phenolics on yeast.
- This analytical approach is valuable for evaluating the adsorption capacity and profile of Saccharomyces cerevisiae towards gallic acid, catechin, and rutin.
- The findings contribute to a better understanding of yeast-grape phenolic interactions in food and fermentation processes.