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Related Experiment Videos

Lactobacilli binding human A-antigen expressed in intestinal mucosa.

Hideaki Uchida1, Hideki Kinoshita, Yasushi Kawai

  • 1Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Miyagi, Japan. hideaki7@bios.tohoku.ac.jp

Research in Microbiology
|April 25, 2006
PubMed
Summary
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Probiotic lactic acid bacteria (LAB) bind to human blood type-A antigens in the gut. This specific binding, identified using biosensor technology, may help these beneficial bacteria colonize the human intestine.

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Lactic acid bacteria (LAB) are crucial for gut health.
  • Understanding LAB adhesion mechanisms is key to their probiotic potential.
  • Human intestinal cells express blood group antigens, which can influence microbial interactions.

Purpose of the Study:

  • To investigate the adherence of human intestinal LAB to blood type-A antigens.
  • To identify specific LAB strains and their surface proteins responsible for A-antigen binding.
  • To explore the potential of A-antigen binding for LAB gut colonization.

Main Methods:

  • Isolation and screening of 93 LAB strains from human feces.
  • Surface plasmon resonance (SPR) biosensor (BIACORE-1000) for binding assays.

Related Experiment Videos

  • SDS-PAGE and N-terminal sequencing to characterize surface layer proteins (SLPs).

    • Main Results:

    • Eleven LAB strains exhibited strong binding to A-trisaccharide probes, with minimal binding to B-trisaccharide probes.
    • Four high-adherence strains were selected for further analysis.
    • The SLP from *Lactobacillus brevis* OLL2772 showed strong A-antigen binding, with homology to known S-layer proteins.

    Conclusions:

    • Specific probiotic LAB strains demonstrate adherence to human blood type-A antigens in the intestinal mucosa.
    • Surface layer proteins, particularly from *Lactobacillus brevis*, are involved in this specific binding.
    • This A-antigen binding capability may facilitate the colonization of probiotic LAB in the human gut.