Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functionally improved bone in calbindin-D28k knockout mice.

David S Margolis1, Devin Kim, John A Szivek

  • 1Orthopaedic Research Lab, Department of Orthopaedic Surgery, University of Arizona, Tucson, AZ 85721, USA.

Bone
|April 25, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuous operation of battery-free implants enables advanced fracture recovery monitoring.

Science advances·2025
Same author

4-Aminopyridine Promotes BMP2 Expression and Accelerates Tibial Fracture Healing in Mice.

The Journal of bone and joint surgery. American volume·2025
Same author

Is it Time to Set a 25% Decrease in eGFR as Criteria for Community-Acquired Acute Kidney Injury in Primary Care Clinics?

The American journal of medicine·2024
Same author

Morphine-induced osteolysis and hypersensitivity is mediated through toll-like receptor-4 in a murine model of metastatic breast cancer.

Pain·2023
Same author

Robot and ultrasound assisted needle insertion to the transverse carpal ligament.

Clinical biomechanics (Bristol, Avon)·2022
Same author

Dose- and time-dependent effects of collagenase clostridium histolyticum injection on transverse carpal ligament elastic modulus and thickness in vitro.

PloS one·2022
Same journal

Baseline β-CTX and BMI predict suitability for deferred zoledronic acid redosing beyond 12 months in postmenopausal Indian women with osteoporosis.

Bone·2026
Same journal

Bone density-based maturation of the midpalatal suture in children aged 8-15 years.

Bone·2026
Same journal

Disrupted phosphate metabolism and SIBLING/ASARM peptide accumulation underlie impaired bone mineralization in klotho-deficient (kl/kl) mice.

Bone·2026
Same journal

Linking genetic variants to bone microstructure: Histological signatures of osteogenesis imperfecta subtypes.

Bone·2026
Same journal

The impact of alcohol consumption on bone mineral density: Insights from cross-sectional and Mendelian randomization studies.

Bone·2026
Same journal

Systemic and local predictors of medication-related osteonecrosis of the jaw in patients receiving antiresorptive therapy: The Shizuoka Kokuho Database study.

Bone·2026
See all related articles

Calbindin-D28k knockout mice exhibit increased bone volume and stiffness, indicating this calcium-binding protein is crucial for regulating bone remodeling. These findings highlight Calbindin-D28k

Area of Science:

  • Bone biology
  • Calcium-binding proteins
  • Osteoporosis research

Background:

  • Calbindin-D28k is a calcium-binding protein involved in osteoblast lifespan and bone mineralization.
  • Understanding Calbindin-D28k's in vivo role requires studying its effects on bone remodeling.
  • Calbindin-D28k knockout (KO) mouse models offer a platform for in vivo investigation.

Purpose of the Study:

  • To investigate the physiological effects of Calbindin-D28k deficiency on bone remodeling in vivo.
  • To characterize bone structure, mineralization, and mechanical properties in Calbindin-D28k KO mice.
  • To determine the role of Calbindin-D28k in regulating bone volume and bone quality.

Main Methods:

  • Histomorphometry and microCT were employed to analyze bone structure and volume.

Related Experiment Videos

  • Bend testing (cantilever and three-point bending) assessed bone mechanical properties.
  • Calbindin-D28k KO mice were compared to wild-type (WT) littermates.

    • Main Results:

    • Calbindin-D28k KO mice showed significantly increased cortical and trabecular bone volume compared to WT mice.
    • Increased bone volume in KO mice was attributed to reduced marrow cavity and endosteal perimeter, and increased trabecular number.
    • KO mice exhibited enhanced bone stiffness and increased failure loads, indicating improved bone strength.
    • Bone formation rates and mineral content (calcium, phosphorus) were similar between KO and WT mice.

    Conclusions:

    • Calbindin-D28k plays a significant role in regulating bone remodeling.
    • Loss of Calbindin-D28k leads to increased bone mass and improved mechanical properties.
    • Calbindin-D28k is a potential therapeutic target for bone diseases characterized by low bone mass.