Related Experiment Video
Updated: Aug 9, 2026
05:45
Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay
Published on: April 5, 2019
[One case report of anti-JK1 antibody undetected on plasma sample]
B Clavier1, R Hadef, C Ait Mansour
1Service d'immunohématologie des receveurs et distribution, centre de transfusion sanguine des armées Jean-Julliard , 1, rue du Lieutenant-Raoul-Batany, BP 410, 92141 Clamart cedex, France. bmoclavier@cegetel.net
Summary
Detecting irregular antibodies is crucial for transfusion safety. A case study revealed plasma samples failed to detect a low-intensity anti-JK1 antibody, highlighting limitations in current detection methods.
Area of Science:
- Transfusion Medicine
- Immunology
- Clinical Pathology
Context:
- Detection of irregular antibodies (DIA) is essential for transfusion safety.
- Standard protocols permit the use of either serum or plasma for DIA testing.
- Irregular antibodies can pose significant risks if undetected.
Purpose:
- To report a case of missed irregular antibody detection in a plasma sample.
- To discuss the challenges associated with detecting and identifying low-intensity antibodies, specifically anti-JK1.
- To evaluate the limitations of current DIA protocols.
Summary:
- A case involving a low-intensity anti-JK1 antibody, detectable in serum, was missed in a plasma sample.
- This antibody is known for being deceptive and potentially dangerous in transfusion settings.
- The findings underscore potential vulnerabilities in DIA procedures when using plasma.
Impact:
- Highlights the need for improved DIA sensitivity and specificity, particularly with plasma samples.
- Informs the refinement of transfusion safety protocols to mitigate risks associated with undetected antibodies.
- Contributes to a better understanding of the limitations and challenges in antibody screening.
