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Fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible
1Laboratory of Virology, Institute of Veterinary Research, Bach mai-Hànôi, Viêtnam.
Abstract:
An immunoperoxidase technique performed on the TGEV-infected cells was developed for detection of virus-induced antigens. The presence of M antigen of TGEV on the surface of infected cells was demonstrated by this technique. This finding is in contrast to the M protein of murine hepatitis coronavirus which migrates to the Golgi apparatus but is not transported to the plasma membrane. The time course of appearance M and S antigens on the surface of TGEV-infected cell can be studied by this technique.
Insights
A new immunoperoxidase technique detects Transmissible Gastroenteritis Virus (TGEV) antigens on infected cell surfaces. This method reveals TGEV M antigen localization, differing from other coronaviruses.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Transmissible Gastroenteritis Virus (TGEV) is a significant pathogen affecting swine.
- Understanding viral antigen localization is crucial for comprehending TGEV infection dynamics.
- Previous studies on other coronaviruses show different M protein localization patterns.
Purpose of the Study:
- To develop and validate an immunoperoxidase technique for detecting TGEV-induced antigens.
- To investigate the surface expression of the TGEV M antigen in infected cells.
- To compare TGEV M antigen localization with that of other coronaviruses.
Main Methods:
- Development of an immunoperoxidase staining technique.
- Application of the technique to TGEV-infected cell cultures.
- Demonstration of viral antigen presence and localization on infected cell surfaces.
Main Results:
- The immunoperoxidase technique successfully detected TGEV-induced antigens.
- The M antigen of TGEV was found on the surface of infected cells.
- This localization contrasts with the Golgi apparatus-restricted M protein of murine hepatitis coronavirus.
Conclusions:
- The developed immunoperoxidase technique is effective for detecting TGEV antigens.
- TGEV M antigen is transported to the plasma membrane of infected cells.
- This technique allows for studying the temporal expression of TGEV M and S antigens on cell surfaces.