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Published on: January 21, 2018
Immunohistochemical findings after LASIK confirm in vitro LASIK model
Siegfried G Priglinger1, Christian-Albrecht May, Claudia S Alge
1Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
Purpose:
To compare immunohistochemical findings in human donor corneas after successful laser in situ keratomileusis (LASIK) without clinical complications with a recently established human LASIK in vitro model.
Methods:
Donor corneas with prior LASIK treatment were investigated. Cryostat sections were stained immunohistochemically for collagen types I, III, and VI and laminin and fibronectin.
Results:
With light microscopy, the interface of the LASIK flap could hardly be detected. In all samples, fibronectin was consistently detected along the entire extent of the surgical wound. In contrast, collagen type III and laminin only stained the superficial portion of the LASIK incision site. Staining for collagen types I and VI showed no changes after LASIK.
Conclusion:
Histologic findings in donor corneas with prior LASIK treatment confirm histologic observations in a recently introduced human organ culture LASIK model. This strengthens the reliability of the latter LASIK model for further studies concerning wound healing after LASIK surgery.
