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[Vitamin C decreases MMP-9 synthesis induced by hydrogen peroxide in an in vitro chorioamniotic membrane model]
César Angel Hernández Guerrero1, María Eugenia Frigolet Vázquez Vela, Tomás Herrerías Canedo
1Departamento de Ultramicroscopía, Instituto Nacional de Perinatología, Lomas Virreyes, México, DF. hehecatlzin@yahoo.com
Objective:
To evaluate the hydrogen peroxide ability to induce the synthesis of matrix metalloproteinase-9 (MMP-9) in an in vitro chorioamniotic membrane model.
Material And Methods:
An experimental, transversal, analytic and prospective study was made. From amniotic membranes (n = 13) of term labour pregnant women chorionic and chorioamniotic explants were prepared; these were stimulated with hydrogen peroxide [200 mcM]. A group of explants were cultured with vitamin C, 48 hours prior to the hydrogen peroxide stimulus. Twenty-four hours after hydrogen peroxide stimulus the supernatants were collected and metalloproteinase-9 production was determined by zymography methods.
Results:
Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C prior to the hydrogen peroxide stimulus, the MMP-9 production diminished (p < 0.05; Dunn's method).
Conclusions:
Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C, 48 hours prior to peroxide hydrogen stimulus, the metalloproteinase-9 production diminished with statistical significance.
Insights
Hydrogen peroxide stimulates matrix metalloproteinase-9 (MMP-9) synthesis in chorioamniotic membranes. Vitamin C pretreatment significantly reduces this MMP-9 production, offering a potential therapeutic avenue.
Area of Science:
- Obstetrics and Gynecology
- Biochemistry
- Cell Biology
Context:
- The chorioamniotic membranes play a crucial role in maintaining pregnancy.
- Oxidative stress, induced by agents like hydrogen peroxide, is implicated in various pregnancy complications.
- Matrix metalloproteinase-9 (MMP-9) is an enzyme involved in tissue remodeling and inflammation.
Purpose:
- To investigate the effect of hydrogen peroxide on the in vitro synthesis of MMP-9 in chorioamniotic membranes.
- To determine if vitamin C can mitigate the hydrogen peroxide-induced MMP-9 production.
Summary:
- Hydrogen peroxide (200 mcM) was used to stimulate chorionic and chorioamniotic explants derived from term labor pregnant women.
- MMP-9 production in the supernatants was quantified using zymography.
- Pre-treatment with vitamin C for 48 hours significantly reduced MMP-9 levels post-hydrogen peroxide stimulation (p < 0.05).
Impact:
- This study demonstrates that hydrogen peroxide induces MMP-9 synthesis in chorioamniotic membranes.
- Vitamin C shows potential as an inhibitor of oxidative stress-induced MMP-9 production in this model.
- Findings may contribute to understanding the role of oxidative stress and MMP-9 in pregnancy pathologies.
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