Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative sequence analysis of human cytomegalovirus strains.

R Lehner1, T Stamminger, M Mach

  • 1Institut fuer Klinische und Molekulare Virologie, Friedrich-Alexander-Universitaet Erlangen/Nuernberg, Germany.

Journal of Clinical Microbiology
|November 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical emergency-complicated infections of the middle ear and paranasal sinuses].

HNO·2026
Same author

Injury patterns of suicide attempts in the head and neck area-a retrospective analysis over 15 years.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2024
Same author

Impact of prenatal hypoxia on the development and behavior of the rat offspring.

Physiological research·2021
Same author

Rare causes of respiratory insufficiency in newborns.

Physiological research·2021
Same author

Impact of perinatal hypoxia on the developing brain.

Physiological research·2020
Same author

Effect of metformin and flutamide on insulin, lipogenic and androgen-estrogen signaling, and cardiometabolic risk in a PCOS-prone metabolic syndrome rodent model.

American journal of physiology. Endocrinology and metabolism·2018
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

Clinical human cytomegalovirus isolates show varied genetic profiles. Some DNA regions are highly conserved, while others exhibit significant variability, allowing for subtype classification.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Human cytomegalovirus (HCMV) is a common pathogen with diverse clinical manifestations.
  • Understanding genetic variability in clinical HCMV isolates is crucial for diagnostics and therapeutics.

Purpose of the Study:

  • To compare DNA sequences of clinical HCMV isolates with laboratory strains.
  • To identify conserved and variable regions within key HCMV genes.

Main Methods:

  • Polymerase chain reaction (PCR) amplification of three DNA regions from five clinical HCMV isolates.
  • Sequence analysis of amplified DNA and predicted amino acid sequences.
  • Comparison with laboratory strains AD169 and Towne.

Main Results:

Related Experiment Videos

  • Nucleotide homologies ranged from 75.8% to 100.0%, and amino acid homologies from 47% to 100%.
  • Observed two patterns: highly conserved regions with scattered mutations (e.g., IE-1/2 promoter, IMP gene) and clusters of high variability (e.g., gp58/116, IMP gene).
  • Variable regions allowed classification of strains into subtypes.

Conclusions:

  • Clinical HCMV isolates exhibit significant genetic diversity.
  • Specific genomic regions demonstrate distinct conservation and variability patterns.
  • Genetic variability may inform HCMV strain classification and understanding of pathogenesis.