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Related Experiment Videos

Adventitial neuronal somata.

Chandra Somasundaram1, Debra I Diz, Timothy Coleman

  • 1Cardiovascular Disease Research Program, Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA. csomasundaram@nccu.edu

Journal of Vascular Research
|April 26, 2006
PubMed
Summary

Researchers discovered novel neuronal cell bodies, termed ANNIES, in rat mesenteric arteries. These adventitial neurons express calcitonin gene-related peptide (CGRP), suggesting a sensory nerve role in these vessels.

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Area of Science:

  • Neuroscience
  • Vascular Biology
  • Gastrointestinal Physiology

Background:

  • The innervation of mesenteric arteries is crucial for regulating blood flow.
  • Previous studies have not definitively identified neuronal cell bodies within the adventitia of these vessels.
  • Calcitonin gene-related peptide (CGRP) is a known marker for certain neuronal populations.

Purpose of the Study:

  • To investigate the presence and characteristics of neuronal structures within rat mesenteric branch arteries (MBA).
  • To determine if beta-CGRP is expressed in the rat MBA.
  • To identify potential neuronal cell bodies in the adventitia of MBA.

Main Methods:

  • Confocal microscopy and immunocytochemistry to visualize nerve fibers and rule out immune cells.

Related Experiment Videos

  • RT-PCR to detect beta-CGRP and alpha-CGRP mRNA expression in mesenteric artery RNA.
  • Cell culture of enzymatically digested arterial segments to isolate and characterize adventitial cells.

    • Main Results:

    • Axonal processes immunostaining for CGRP were observed in whole-mount MBAs.
    • RT-PCR confirmed the expression of beta-CGRP mRNA in the mesenteric artery arcade.
    • A subset of cultured adventitial cells sprouted axons and immunostained for neuronal markers and CGRP, distinct from fibroblasts and smooth muscle cells.

    Conclusions:

    • This study provides the first evidence for a population of adventitial neuronal somata (ANNIES) in small mesenteric arteries.
    • These ANNIES express CGRP and may originate from sensory nerves.
    • The findings suggest a previously unrecognized intrinsic innervation of mesenteric arteries.