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Related Experiment Videos

Comparison of methods for counting cells in the mouse glomerulus.

John M Basgen1, Susanne B Nicholas, Michael Mauer

  • 1Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.

Nephron. Experimental Nephrology
|April 26, 2006
PubMed
Summary

The disector/fractionator method accurately estimates glomerular cell counts, serving as a practical alternative to the laborious Exhaustive Count method. The Weibel-Gomez method, however, tends to overestimate cell numbers.

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Area of Science:

  • Nephrology
  • Stereology
  • Quantitative Biology

Background:

  • Accurate glomerular cell counting is crucial but challenging.
  • Existing methods like Exhaustive Count, disector/fractionator, and Weibel-Gomez yield conflicting results.
  • A practical and accurate method is needed to replace time-consuming techniques.

Purpose of the Study:

  • To compare the accuracy and practicality of the disector/fractionator and Weibel-Gomez methods for estimating glomerular cell number.
  • To identify a reliable alternative to the Exhaustive Count method.

Main Methods:

  • Glomeruli from normal mouse kidneys were sectioned and imaged.
  • Cell counts were performed using the Exhaustive Count, disector/fractionator, and Weibel-Gomez methods.
  • Statistical analysis was used to compare the results.

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Main Results:

  • The disector/fractionator method yielded 211 +/- 29 cells/glomerulus, not statistically different from the Exhaustive Count method (213 +/- 22 cells/glomerulus, p = 0.82).
  • The Weibel-Gomez method yielded 235 +/- 26 cells/glomerulus, significantly different from the Exhaustive Count method (p = 0.003).
  • The Weibel-Gomez method resulted in a 10% overestimation.

Conclusions:

  • The disector/fractionator method is an unbiased and practical substitute for the Exhaustive Count method.
  • The Weibel-Gomez method overestimates glomerular cell number and is less suitable.
  • Accurate stereological methods are essential for reliable cell counting in kidney research.