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A human anti-Jkb monoclonal antibody
M Lecointre-Coatmelec1, D Bourel, J Ferrette
1Centre Régional de Transfusion Sanguine, Rennes, France.
Vox Sanguinis
|January 1, 1991
Summary
Researchers developed a specific monoclonal antibody for Jkb blood grouping. This antibody, derived from hybridoma technology, shows potential as a reliable blood grouping reagent.
Area of Science:
- Immunology
- Cell Biology
- Serology
Background:
- Monoclonal antibodies are crucial for precise immunological assays.
- Accurate blood grouping is essential for safe transfusions.
- The Jkb blood group antigen requires specific detection methods.
Purpose of the Study:
- To develop a hybridoma cell line secreting anti-Jkb monoclonal antibody.
- To produce and characterize the monoclonal antibody for blood grouping applications.
Main Methods:
- Peripheral blood mononuclear cells from an immunized patient were used.
- Cells were transformed with Epstein-Barr virus and fused with P3X63Ag8 myeloma cells.
- Hybridoma cloning and antibody supernatant production were performed.
Main Results:
- A hybridoma cell line secreting specific anti-Jkb monoclonal antibody was successfully isolated.
- The produced monoclonal antibody was tested as a blood grouping reagent.
Conclusions:
- The isolated monoclonal antibody is a potential candidate for Jkb blood grouping.
- This development offers a new tool for serological testing and transfusion compatibility.