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Related Experiment Videos

Antigen-stimulated changes in cyclic nucleotide levels in the mouse.

I Yamamoto, D R Webb

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1975
    PubMed
    Summary

    Mice injected with sheep red blood cells (sRBC) show rapid increases in splenic cyclic adenosine monophosphate (cAMP) and sustained elevations in cyclic guanosine monophosphate (cGMP). These immune responses depend on T lymphocytes and prostaglandin synthesis.

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    Area of Science:

    • Immunology
    • Cellular Signaling

    Background:

    • Cyclic nucleotides like cAMP and cGMP play crucial roles in cellular communication and immune responses.
    • Understanding the dynamics of cyclic nucleotides during immune activation is vital for deciphering immune cell function.

    Purpose of the Study:

    • To investigate the in vivo changes in splenic cyclic nucleotide levels following intravenous injection of sheep erythrocytes (sRBC).
    • To determine the role of T lymphocytes, prostaglandin synthesis, and antigen specificity in these cyclic nucleotide alterations.

    Main Methods:

    • Intravenous injection of sRBC into mice.
    • Measurement of splenic cAMP and cGMP levels at various time points post-injection.
    • Assessment of cyclic nucleotide changes in athymic nude mice and following cortisone acetate pretreatment.

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  • Evaluation of the effects of indomethacin and autonomic nervous system blockers.

    • Main Results:

    • sRBC injection caused a rapid, transient increase in splenic cAMP and a delayed, sustained increase in splenic cGMP.
    • These changes were dependent on thymus-derived (T) lymphocytes and prostaglandin synthesis.
    • Secondary sRBC stimulation induced a greater cAMP response, indicating immunological memory.
    • Soluble protein antigens did not elicit similar early cyclic nucleotide changes.

    Conclusions:

    • Splenic cyclic nucleotide dynamics, particularly cAMP and cGMP, are integral to T-cell-mediated immune responses to particulate antigens like sRBC.
    • Prostaglandin synthesis and T-cell presence are critical for modulating these cyclic nucleotide responses.
    • The findings correlate in vivo observations with known in vitro immune cell signaling pathways.