Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measuring tissue-based biomarkers by immunochromatography coupled with reverse-phase lysate microarray.

Martin J Romeo1, John Wunderlich, Lien Ngo

  • 1Laboratory of Pathology, Surgery Branch, and Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|April 28, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory Challenges and Opportunities for Human Studies Using Archival Tissue.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

<i>Trichosporon inkin</i> meningitis in a pediatric patient diagnosed via metagenomic sequencing.

ASM case reports·2026
Same author

Imaging Mass Cytometry Immune Profiling of Hunner Lesions in a Convenience Sample of Patients With Interstitial Cystitis/Bladder Pain Syndrome.

The Journal of urology·2025
Same author

International Society of Urological Pathology Consensus on Cancer Precursor Lesions. Working Group 1: The Prostate.

The American journal of surgical pathology·2025
Same author

Sox9 in the epicardium: Implications for cell invasion, differentiation, and coronary vascular development.

PloS one·2025
Same author

Fes-Deficient Macrophages Prime CD8+ T Cells to Stimulate Antitumor Immunity and Improve Immunotherapy Efficacy.

Cancer research·2025
Same journal

Clinical Validation of a Multiplex Urine Biomarker Assay for Surveillance of Recurrent Bladder Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same journal

Epigenetic Liquid Biopsy Enables Universal Mutation-Agnostic Molecular Surveillance for High-Risk Neuroblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same journal

Claudin 18.2 Targeting: A Pan-Cancer Perspective.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same journal

High-Sensitivity ctDNA Analysis Uncovers Relevant Signals Missed by NGS in Pancreatic Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same journal

Pediatric Brain Tumor Consortium phase 1 study of CD40 agonist sotigalimab in pediatric and young adult patients with recurrent CNS tumors and newly-diagnosed DIPG.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same journal

A randomized phase 2 study of combination atezolizumab and varlilumab (CDX-1127) with or without cobimetinib in previously-treated unresectable biliary tract cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
See all related articles

A new method, immunochromatography coupled with reverse-phase lysate microarrays (I-RPM), offers reproducible tissue biomarker quantification. I-RPM shows strong correlations with other methods for gp100 and MART-1, validating its potential.

Area of Science:

  • Biomarker Discovery
  • Translational Research
  • Cancer Diagnostics

Background:

  • Immunohistochemistry (IHC) is limited by variability and bias.
  • Alternative technologies like RT-PCR, ICC, and RPM require validation.
  • RPM faces challenges with microdissection and low protein yield.

Purpose of the Study:

  • To validate and correlate novel biomarker technologies on patient tissues.
  • To assess the utility of immunochromatography coupled with RPM (I-RPM) for biomarker analysis.
  • To overcome limitations of current tissue biomarker assessment methods.

Main Methods:

  • Surgically excised metastatic melanoma from 30 patients.
  • Specimens processed for IHC, ICC, RT-PCR, and I-RPM.
  • I-RPM utilized immunochromatography for melanoma cell enrichment.

Related Experiment Videos

  • Expression of gp100 and MART-1 was measured and normalized to actin.

    • Main Results:

    • I-RPM demonstrated reproducibility (r ≥ 0.70).
    • I-RPM correlated strongly with IHC and ICC for gp100 (r=0.78, 0.76).
    • I-RPM showed moderate correlation with RT-PCR for gp100 (r=0.61).
    • I-RPM correlated strongly with RT-PCR for MART-1 (r=0.78).
    • Transcript levels showed moderate correlations with IHC/ICC (r=0.41-0.64).

    Conclusions:

    • I-RPM is a promising technology for quantitative biomarker grading.
    • The method overcomes limitations of traditional techniques.
    • Antigen-dependent correlations highlight the need for careful validation.