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Updated: Aug 9, 2026
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Kidney Procurement in a Preclinical Large Animal Model
Published on: May 2, 2025
Development of a technique for serial bilateral renal biopsy in steers
O Alberto Chiesa1, Richard Cullison, David E Anderson
1Center for Veterinary Medicine, Office of Research, Division of Residue Chemistry, Food & Drug Administration, 8401 Muirkirk Road, Laurel, Maryland 20708, USA. ochiesa@cvm.fda.gov
Summary
A novel laparoscopic biopsy technique allows serial kidney sampling in standing steers. This minimally invasive method provides valuable renal tissue for research with minimal adverse effects in most animals.
Area of Science:
- Veterinary Surgery
- Nephrology
- Animal Science
Background:
- Serial kidney sampling is crucial for pharmacokinetic and toxicological studies in livestock.
- Minimally invasive techniques are preferred to reduce animal stress and improve welfare.
Purpose of the Study:
- To develop and evaluate a laparoscopic biopsy procedure for obtaining serial renal samples from standing steers.
- To assess the feasibility and safety of repeated kidney biopsies in cattle.
Main Methods:
- Laparoscopic surgery was performed on standing steers under sedation and local anesthesia.
- Renal biopsy forceps were used via trocar incisions for sample collection under endoscopic guidance.
- Physiological parameters and clinical signs were monitored post-procedure.
Main Results:
- The procedure successfully yielded serial renal samples (2-3 mm diameter) from 10 steers.
- 48 renal samples were collected across 51 procedures with no significant clinical changes in 9 steers.
- One steer experienced fatal hemorrhage after the third biopsy, highlighting a potential risk.
Conclusions:
- Laparoscopic renal biopsy is a viable technique for serial tissue acquisition in standing steers.
- The procedure is generally safe, but careful technique is required to mitigate risks like hemorrhage.
