Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Singlepath Salmonella. Performance-Tested Method 060401.

Lisa Thompson1, Charlotte Lindhardt

  • 1Merck Chemicals Ltd., Newport Technical Centre, 5 The Courtyard, Imperial Park, Newport NP10 8UL, Wales, United Kingdom. lisa.thompson@merckchem.co.uk

Journal of AOAC International
|April 28, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In the Nick of Time: Same-Day Simulation-Paired Procedure Clinic for Internal Medicine Residents.

Journal of graduate medical education·2026
Same author

Patient, Family Caregiver and Health Care Provider Experiences With a Hospital at Home Program in British Columbia, Canada.

Quality management in health care·2026
Same author

Patient reported barriers and facilitators of chronic kidney disease care in Sub-Saharan Africa: a mixed methods systematic review using the COM-B model.

BMC nephrology·2025
Same author

Measuring growth in research capacity and capability of allied health professionals at a regional Australian public health service: 2011-2023.

BMC health services research·2025
Same author

Development and evaluation of a tool (named Evidence Brief) to communicate allied health research translation.

BMC health services research·2025
Same author

Evaluation of salivary urea nitrogen test in cats with lower urinary tract outflow obstructions.

The Canadian veterinary journal = La revue veterinaire canadienne·2025
Same journal

Single-Laboratory Validation of Simultaneous Determination of Aflatoxins in Nutraceuticals following immune-affinity Column Cleanup and Liquid Chromatography Tandem Mass Spectrometry Analysis.

Journal of AOAC International·2026
Same journal

Determination of Bromoform in Seaweed, Oil, and Animal Feed by GC-MS/MS: AOAC Official Method 2026.01, First Action.

Journal of AOAC International·2026
Same journal

ICRF-Assisted Box-Behnken Design and Optimization for Rapid UPLC-PDA Determination of Dorzolamide Hydrochloride and Timolol Maleate in an Ophthalmic Preparation.

Journal of AOAC International·2026
Same journal

Advancing PFAS Analysis Through Scientific Publication.

Journal of AOAC International·2026
Same journal

A Green UPLC-UV Method for L-Ascorbic Acid Determination Based on a Biodegradable Chelating Agent and Synergistic Hydrophobic-Electrostatic Interactions.

Journal of AOAC International·2026
Same journal

Quantification of 24 Inorganic Impurities in Vismodegib and Idelalisib Anti-Cancer Drug Substances by Using ICP-MS.

Journal of AOAC International·2026
See all related articles

Singlepath Salmonella is an effective lateral flow assay for detecting Salmonella in various foods. This method showed no significant difference compared to the ISO reference, with acceptable false-positive and false-negative rates.

Area of Science:

  • Food safety and microbiology
  • Diagnostic assay development
  • Bacteriology

Background:

  • Salmonella spp. are significant foodborne pathogens requiring reliable detection methods.
  • Immunochromatographic assays offer rapid presumptive detection of bacterial pathogens in food matrices.

Purpose of the Study:

  • To evaluate the performance of the Singlepath Salmonella assay for the presumptive qualitative detection of Salmonella spp. in various food types.
  • To compare the Singlepath Salmonella assay against the ISO 6579:2002 reference method.

Main Methods:

  • The Singlepath Salmonella assay, a lateral flow immunoassay, was used for presumptive Salmonella detection.
  • AOAC Performance-Tested Method study design was employed, including inclusivity and exclusivity testing.
  • Foods were artificially inoculated with Salmonella spp. at varying concentrations and compared to the ISO 6579:2002 reference method.

Related Experiment Videos

Main Results:

  • The Singlepath Salmonella assay demonstrated effectiveness in detecting Salmonella spp. in dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey.
  • No significant difference was observed between the Singlepath Salmonella assay and the ISO 6579:2002 reference method (Chi-square = 0.9).
  • The assay exhibited a 7.3% false-positive rate and a 2.5% false-negative rate, with 100% inclusivity for tested Salmonella serovars and no cross-reactivity with 58 non-Salmonella species.

Conclusions:

  • Singlepath Salmonella is a reliable and effective method for the presumptive qualitative detection of Salmonella spp. in a range of food products.
  • The assay's performance is comparable to the established ISO reference method, making it a viable option for food safety testing.
  • The high inclusivity and specificity of the Singlepath Salmonella assay support its use in routine food microbiology laboratories.