Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Processing body autoantibodies reconsidered.

Donald B Bloch, Tod Gulick, Kenneth D Bloch

    RNA (New York, N.Y.)
    |April 28, 2006
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of Continuous Low-dose Nitric Oxide in a Murine Model of Pulmonary Hypertension with Impaired Lung Development.

    Anesthesiology·2026
    Same author

    Focal Adhesion Kinase Promotes Calcification of Vascular Smooth Muscle Cells via Regulation of Histone Deacetylase 4 and 5.

    Arteriosclerosis, thrombosis, and vascular biology·2025
    Same author

    CYR119, a central nervous system-penetrant stimulator of soluble guanylyl cyclase, improves survival in a mouse model of resuscitation after cardiac arrest.

    Nitric oxide : biology and chemistry·2025
    Same author

    Prolonged Postnatal Hypoxia Impairs Lung Development and Causes Severe Pulmonary Hypertension in Mice.

    Journal of the American Heart Association·2025
    Same author

    Analysis of Methadone-Related Poisoning Cases.

    Fa yi xue za zhi·2025
    Same author

    Screen of the ReFRAME Compound Library for Therapeutic Agents to Prevent Red Blood Cell Sickling Using an Improved High Throughput Sickling Assay.

    ACS omega·2025
    Same journal

    Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

    RNA (New York, N.Y.)·2026
    Same journal

    SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

    RNA (New York, N.Y.)·2026
    Same journal

    Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

    RNA (New York, N.Y.)·2026
    Same journal

    Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

    RNA (New York, N.Y.)·2026
    Same journal

    RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

    RNA (New York, N.Y.)·2026
    Same journal

    EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

    RNA (New York, N.Y.)·2026
    See all related articles

    Autoimmune disease sera targeting P-bodies are unreliable GW182 markers. These sera contain antibodies against Ge-1, a different P-body autoantigen, complicating research on mRNA regulation.

    Area of Science:

    • Cell biology
    • Immunology
    • Molecular genetics

    Background:

    • Processing bodies (P-bodies) are crucial for mRNA degradation and gene silencing.
    • Autoimmune diseases can feature antibodies against P-bodies.
    • GW182 is a known P-body component used as a target for autoantibodies.

    Purpose of the Study:

    • To evaluate the reliability of existing reference sera as markers for the GW182 protein in P-bodies.
    • To identify potential cross-reactivity in autoantibodies directed against P-body components.

    Main Methods:

    • Immunofluorescence assays to detect autoantibodies in patient sera.
    • Characterization of autoantigens targeted by these antibodies.
    • Comparison of antibody reactivity against GW182 and other P-body proteins.

    Related Experiment Videos

    Main Results:

    • Reference sera previously used to detect GW182 showed cross-reactivity.
    • These sera contain antibodies targeting Ge-1, a distinct P-body autoantigen.
    • The presence of anti-Ge-1 antibodies makes GW182 detection unreliable with these sera.

    Conclusions:

    • Available reference sera are not specific markers for GW182 due to anti-Ge-1 antibodies.
    • Re-evaluation of autoantibody specificities is necessary for accurate P-body research.
    • This finding impacts studies of autoimmune diseases and post-transcriptional gene regulation.