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Nuclear targeting sequences--a consensus?

C Dingwall1, R A Laskey

  • 1Wellcome Trust/Cancer Research Campaign Institute of Cancer and Development Biology, Cambridge.

Trends in Biochemical Sciences
|December 1, 1991
PubMed
Summary

Nuclear targeting sequences guide proteins into the cell nucleus. Despite diverse structures, a common bipartite motif is identified across various nuclear localization signals.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Nuclear targeting sequences (NTS) mediate protein import into the nucleus.
  • The SV40 large T antigen NTS is a widely accepted model.
  • However, diverse NTS structures exist, complicating nuclear transport understanding.

Purpose of the Study:

  • To review the diversity of nuclear targeting sequences.
  • To identify a unifying motif within these sequences.
  • To propose a consensus bipartite motif for nuclear localization.

Main Methods:

  • Literature review of nuclear targeting sequences.
  • Comparative analysis of known NTS.
  • Bioinformatic analysis to identify conserved motifs.

Main Results:

  • Nuclear targeting sequences exhibit significant structural diversity.
  • A conserved bipartite motif is identified across various NTS.
  • This bipartite motif is proposed as a consensus for nuclear localization.

Conclusions:

  • Despite diversity, a consensus bipartite motif underlies nuclear targeting.
  • This finding simplifies the understanding of nuclear protein import.
  • The consensus motif has implications for protein engineering and drug delivery.

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