Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Blockade of VEGF accelerates proteinuria, via decrease in nephrin expression in rat crescentic glomerulonephritis.

A Hara1, T Wada, K Furuichi

  • 1Department of Gastroenterology and Nephrology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.

Kidney International
|April 28, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transport of fragmented DNA in apical dendrites of gerbil CA1 pyramidal neurons following transient forebrain ischemia.

Brain research·1998
Same author

Enhancement of peroxynitrite-evoked acetylcholine release by hydroxyl radical scavengers from mouse cerebral cortical neurons.

Life sciences·1998
Same author

Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA.

The Biochemical journal·1998
Same author

Protective effect of apoptosis-inhibitory agent, N-tosyl-L-phenylalanyl chloromethyl ketone against ischemia-induced hippocampal neuronal damage.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·1998
Same author

Inhibitory effects of nabumetone, a cyclooxygenase-2 inhibitor, and esculetin, a lipoxygenase inhibitor, on N-methyl-N-nitrosourea-induced mammary carcinogenesis in rats.

Japanese journal of cancer research : Gann·1998
Same author

Serum soluble CD44 levels for monitoring disease states in acute leukemia and myelodysplastic syndromes.

International journal of oncology·1998

Inhibition of vascular endothelial growth factor (VEGF) worsened kidney disease progression in rats, leading to increased proteinuria and vascular damage. This highlights VEGF

Area of Science:

  • Nephrology
  • Molecular Biology
  • Angiogenesis Research

Background:

  • Vascular endothelial growth factor (VEGF) is crucial for maintaining kidney vascular networks.
  • Soluble fms-like tyrosine kinase 1 (sFlt-1) inhibits VEGF activity by binding circulating VEGF.
  • Progressive glomerulonephritis (GN) involves damage to the kidney's filtration barrier and vasculature.

Purpose of the Study:

  • To investigate the role of VEGF in maintaining kidney function and structure during progressive glomerulonephritis (GN).
  • To determine the effects of VEGF inhibition on glomerular filtration barrier integrity and renal vascularization in a rat model of GN.

Main Methods:

  • Rats with anti-glomerular basement membrane antibody-induced GN were treated with sFlt-1 to inhibit VEGF activity.

Related Experiment Videos

  • VEGF activity was blocked by transfecting rats with plasmid DNA encoding the murine sFlt-1 gene.
  • Urinary protein excretion, nephrin expression, macrophage infiltration, crescent formation, glomerulosclerosis, interstitial fibrosis, renal function, and peritubular capillary (PTC) loss were assessed.

    • Main Results:

    • VEGF inhibition with sFlt-1 led to massive proteinuria and decreased nephrin expression in nephritic rats.
    • Blockade of VEGF also induced mild proteinuria in normal rats.
    • sFlt-1 treatment accelerated glomerulosclerosis, interstitial fibrosis, renal dysfunction, and peritubular capillary (PTC) loss in GN rats, without affecting macrophage infiltration or crescent formation.

    Conclusions:

    • VEGF plays a vital role in preserving podocyte function and maintaining renal vasculature.
    • Inhibition of VEGF exacerbates kidney injury, promoting glomerulosclerosis and fibrosis.
    • VEGF is protective against progressive renal insults in the kidney.