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Updated: Aug 9, 2026
Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
Published on: September 16, 2018
[Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63]
1Department of Hepatitis Virus, Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Background:
To study a new kind of adjuvant: transcutaneous immunization adjuvant.
Methods:
The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.
Results:
The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.
Conclusion:
The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.
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