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Updated: Aug 8, 2026

A Fluorescence-based Assay of Phospholipid Scramblase Activity
Published on: September 20, 2016
Identification of an erythroid ATP-dependent aminophospholipid transporter
Eric Soupene1, Frans A Kuypers
1Children's Hospital Oakland Research Institute, Martin Luther King Jr Way, Oakland, CA 94609, USA. esoupene@chori.org
Abstract:
The asymmetric distribution of amino-containing phospholipids in plasma membranes is essential for the function and survival of mammalian cells. Phosphatidylserine (PS) is restricted to the inner leaflet of plasma membranes by an ATP-dependent transport process. Exposure of PS on the surface of cells serves as a binding site for haemostatic factors, triggers cell-cell interaction and recognition by macrophages and phospholipases. Exposure of PS on the red cell surface plays a significant role in sickle cell pathology. We report the identification of two different isoforms of the aminophospholipid translocase, Atp8a1, or flippase, in the murine red blood cell membrane.
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