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Updated: Aug 9, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
[Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method]
Xiao-ying Guo1, Yong-ning Wang, Lin-gang Gu
1State Key Laboratory for Bioelectronics, Southeast University, Nanjing 210096, China.
Aim:
To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food.
Methods:
The assay was established based on the alkaline phosphatase (ALP) labeled anti-E. coli O157:H7 antibody, which reacted with an excellent chemiluminescent reagent, 3(2' spiroadamantane)4 methoxy 4(3''phosphoryloxy) phenyl 1,2 dioxetane (AMPPD) buffer solution and emitted the photons.
Results:
The sensitivity of chemiluminescence assay was 850, and the linear range was 1000-50,000. The intra- and interassay CV values (CVs) were respectively below 15% and 20%. The correlation coefficient was 0.9807 by comparing with the conventional counting method.
Conclusion:
The chemiluminescent immunoassay is more accurate than other methods in the detection of concentration of E. coli O157:H7 in samples.
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