Related Experiment Video
Updated: Aug 9, 2026
Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents
Published on: June 8, 2022
Test-retest reproducibility of 50 kHz linear-electrical impedance myography
Seward B Rutkove1, Kyungmouk S Lee, Carl A Shiffman
1Division of Neuromuscular Diseases, Department of Neurology, Harvard Medical School, Beth Israel Deaconess Medical Center, 330 Brookline Ave, TCC 810, Boston, MA 02215, USA. srutkove@bidmc.harvard.edu
Objective:
Electrical impedance myography (EIM) is a method for evaluating muscle in which high-frequency, low-intensity alternating current is applied to a body region and the resulting surface voltage pattern over a muscle of interest is measured. In this study, the reproducibility for the simplest of these techniques, 50 kHz linear-EIM, was assessed for three muscles.
Methods:
Fifty kilohertz linear-EIM was performed on the biceps, quadriceps, and tibialis anterior of 30 normal subjects ranging in age from 21 to 90 years, and the major outcome variable, the spatially averaged phase (thetaavg), measured. The measurements were repeated within 250 days and comparisons between the two data sets made.
Results:
Reproducibility, as measured by the intraclass correlation coefficients for all three muscles, was very high at 0.970, 0.971, and 0.938 for biceps, quadriceps, and tibialis anterior, respectively. Variability between measurements was on average 4.2% for all muscle combined, with an upper limit of 16.8%.
Conclusions:
Fifty kilohertz linear-EIM demonstrates excellent test-retest reproducibility.
Significance:
These results support the view that 50 kHz linear-EIM has the potential to be used as a simple, fast, and non-invasive measurement for the assessment of disease status, either as part of individual patient care or as a surrogate outcome measure in clinical trials work.
