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Published on: July 15, 2011
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
J P Singh1, Rishendra Verma, P Chaudhuri
1Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India.
Abstract:
The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied.
