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The phagocyte oxidative metabolism function in ankylosing spondylitis.
D Wendling1, J M Didier, D A Vuitton
1Service de Rhumatologie, Centre Hospitalier Régional et Universitaire, Besançon, France.
Rheumatology International
|January 1, 1991
Summary
Phagocyte oxidative metabolism is significantly higher in ankylosing spondylitis (AS) patients compared to controls. This suggests an activated myeloperoxidase system in AS, regardless of HLA-B27 status.
Area of Science:
- Immunology
- Rheumatology
- Cellular Biology
Background:
- Ankylosing spondylitis (AS) is a chronic inflammatory disease.
- Phagocyte function is crucial in immune responses.
- Oxidative metabolism in phagocytes plays a role in inflammation.
Purpose of the Study:
- To investigate phagocyte oxidative metabolism in ankylosing spondylitis (AS) patients.
- To compare phagocyte function in AS patients with healthy controls.
- To explore the role of the myeloperoxidase system in AS.
Main Methods:
- Chemiluminescence assay was used to measure phagocyte oxidative metabolism in whole blood.
- Measurements were taken from resting and stimulated cells (latex, zymosan, fMLP).
- Luminol and lucigenin were used as chemiluminescence amplifiers.
Main Results:
- Maximal light intensity, indicating oxidative metabolism, was significantly higher in AS patients (P < 0.01) compared to controls.
- This increase was observed in both resting and stimulated phagocytes.
- No significant difference was found between HLA-B27 positive and negative AS patients.
Conclusions:
- Phagocyte oxidative metabolism is enhanced in ankylosing spondylitis.
- The findings suggest an activation of the myeloperoxidase system in AS patients.
- The observed changes in phagocyte function are independent of HLA-B27 status.