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Related Experiment Videos

Sample pretreatment on a microchip with an integrated electrospray emitter.

Peter Lindberg1, Andreas P Dahlin, Sara K Bergström

  • 1Department of Analytical Chemistry, Uppsala University, Sweden.

Electrophoresis
|April 29, 2006
PubMed
Summary

This study introduces a novel microbead-packed polydimethylsiloxane (PDMS) microchip for sample pretreatment before sheathless electrospray ionization mass spectrometry (ESI-MS). The device efficiently desalted and enriched neuropeptides, demonstrating long-term stability and high sensitivity.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Microfluidics

Background:

  • Microfluidic devices offer miniaturized platforms for chemical analysis.
  • Sample pretreatment, including desalting and enrichment, is crucial for sensitive mass spectrometry.
  • Integrating multiple analytical functions on a single chip simplifies workflows.

Purpose of the Study:

  • To develop and demonstrate a polydimethylsiloxane (PDMS) microchip with integrated electrospray emitter for sample pretreatment.
  • To prove the concept of integrating analytical functions on a centimeter-sized chip.
  • To achieve sheathless electrospray ionization mass spectrometry (ESI-MS) for enhanced sensitivity.

Main Methods:

  • Fabrication of PDMS microchip substrates using SU-8 masters and corona discharge bonding.

Related Experiment Videos

  • Packing the microchip channel with 5-micrometer hypercross-linked polystyrene beads for sample trapping.
  • Coating the integrated emitter with graphite powder for electrical contact in sheathless ESI.
  • Performing sample desalting and enrichment of neuropeptides using the packed beads.

    • Main Results:

    • Demonstration of a stable, continuous electrospray from the microchip for over 800 hours.
    • Successful desalting and enrichment of neuropeptides from physiological salt solutions.
    • Achieved a limit of detection (LOD) of approximately 20 femtomoles for angiotensin II using time-of-flight mass spectrometry (TOF MS).

    Conclusions:

    • The developed PDMS microchip effectively integrates sample pretreatment functions for sheathless ESI-MS.
    • The device exhibits excellent durability and long-term operational stability.
    • This approach enables sensitive analysis of neuropeptides with simplified sample handling.