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Updated: Aug 9, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Progress in capillary electrophoresis coupled to matrix-assisted laser desorption/ionization - time of flight mass
Christian W Huck1, Rania Bakry, Lukas A Huber
1Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria. Christian.W.Huck@uibk.ac.at
Abstract:
In this review the most important techniques developed to hyphenate CE to MALDI-TOF-MS are summarized. The principles of the different interfaces and ways to solve the hyphenation problem are explained and discussed in detail. The most important applications especially in the proteomic field are reviewed, and the advantages of CE-MALDI-TOF-MS for the analysis of these compounds compared to other techniques such as ESI-MS are exhaustingly discussed from a critical point of view. CE coupled to MALDI-TOF-MS has started to overpass traditionally used CE-coupling techniques, especially CE-ESI-MS, offering the possibility to analyze samples of interest even weeks after CE analysis and using multiplexing systems for high-sample throughput.
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