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KIAA0101 (OEACT-1), an expressionally down-regulated and growth-inhibitory gene in human hepatocellular carcinoma

Minglei Guo1, Jinjun Li, Dafang Wan

  • 1Shanghai Medical College, Fudan University, Shanghai, China. guomingleicn@yahoo.com.cn

BMC Cancer
|May 2, 2006
PubMed
Abstract

Insights

KIAA0101 protein is down-regulated in hepatocellular carcinoma (HCC). This gene inhibits HCC cell growth and progression by blocking the cell cycle.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Previous cDNA array studies identified KIAA0101 as differentially expressed in human hepatocellular carcinoma (HCC).
  • Further investigation at the protein level and functional analysis in HCC is crucial.

Purpose of the Study:

  • To investigate KIAA0101 protein expression in HCC versus non-cancerous liver tissues.
  • To determine the subcellular localization of KIAA0101.
  • To elucidate the role of KIAA0101 in HCC cell growth and cell cycle progression.

Main Methods:

  • Western blot and tissue array analysis of paired HCC and non-cancerous liver tissues.
  • Subcellular localization determined by dual fluorescence imaging and mitochondrial protein analysis.
  • In vitro cell growth curves and FACS analysis of HCC cells with KIAA0101 overexpression.

Main Results:

  • KIAA0101 protein was down-regulated in 25/30 HCC tissues compared to non-cancerous tissues.
  • Tissue array confirmed down-regulation in 161 paired samples.
  • KIAA0101 localized to mitochondria and nuclei, inhibited HCC cell growth, and arrested cells at the G1 to S phase transition.

Conclusions:

  • KIAA0101 protein expression is significantly reduced in HCC.
  • KIAA0101 exhibits tumor-suppressive properties by inhibiting HCC cell proliferation.
  • The mechanism involves cell cycle arrest at the G1 to S phase transition.

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