Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modeling Hsp70-mediated protein folding.

Bin Hu1, Matthias P Mayer, Masaru Tomita

  • 1Institute for Advanced Biosciences, and Graduate School of Media and Governance, Keio University, Tsuruoka, Japan. hubin@sfc.keio.ac.jp

Biophysical Journal
|May 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restricted accessed material-copper(II) ion imprinted polymer solid phase extraction combined with inductively coupled plasma-optical emission spectrometry for the determination of free Cu(II) in urine and serum samples.

Talanta·2013
Same author

Spin radical enhanced magnetocapacitance effect in intermolecular excited states.

The journal of physical chemistry. B·2013
Same author

Recent developments in stir bar sorptive extraction.

Analytical and bioanalytical chemistry·2013
Same author

Discovery of MK-8742: an HCV NS5A inhibitor with broad genotype activity.

ChemMedChem·2013
Same author

Magnetic polycarbonate microspheres for tumor-targeted delivery of tumor necrosis factor.

Drug delivery·2013
Same author

A study on validity of cortical alpha connectivity for schizophrenia.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
See all related articles

Computer simulations reveal the Hsp70 chaperone system

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Computational Biology

Background:

  • The Hsp70 chaperone system is crucial for protein folding in all cells.
  • Understanding its kinetic mechanisms is essential for comprehending cellular protein homeostasis.

Purpose of the Study:

  • To analyze the kinetic characteristics of the Escherichia coli Hsp70 chaperone system during protein refolding.
  • To compare simulation results with in vitro refolding experiments.

Main Methods:

  • Utilized computer simulations to model the Hsp70 chaperone system's kinetics.
  • Derived and scrutinized rate constants from literature and experimental data.
  • Compared simulation outcomes with in vitro refolding experiments of wild-type and mutant proteins.

Related Experiment Videos

Main Results:

  • Simulation results align with reported laboratory experiments for both wild-type and mutant proteins.
  • Demonstrated the robustness of the Hsp70 system in assisting protein folding across varied kinetic parameters and component concentrations.
  • Highlighted the critical role of synergistic stimulation of Hsp70's ATPase activity.

Conclusions:

  • The developed kinetic model accurately simulates Hsp70-assisted protein refolding.
  • Identified limitations in the current model, pointing to areas needing further research in chaperone mechanisms.
  • The model serves as a foundation for future investigations into chaperone action and protein refolding.