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Updated: Aug 9, 2026
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
Published on: January 11, 2013
Microdialysis measurement of acetylcholine in rat hippocampus during severe insulin-induced hypoglycemia
Hiroyuki Umegaki1, Yoko Yamaguchi, Yusuke Suzuki
1Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsuruma-Cho, Showa-Ku, Nagoya, Aichi 466-8550, Japan. umegaki@med.nagoya-u.ac.jp
Objective:
It has been well acknowledged that hypoglycemia induces cognitive impairment. However, the behavior of hippocampal cholinergic system, which is thought to play a key role in cognition, during hypoglycemia remains to be elucidated.
Materials And Methods:
In this study we conducted a microdialysis using 9 weeks old virgin male Wistar/ST rats to determine the changes of extracelluar acethylcholine and glucose levels in the hippocampus during insulin-induced severe hypoglycemia, and compared the results with those obtained from a microdialysis in the striatum.
Results:
The extracelluar concentrations of acethylcholine both in the hippocampus and striatum did not change during hypoglycemia.
Conclusion:
The results of the present study may suggest that changes of hippocampal cholinergic release may not be involved in the mechanism of cognitive impairment during hypoglycemia.
