Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

2.0K
This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

10.0K
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

14.5K
The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
14.5K
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

9.2K
This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
9.2K
Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

39.1K
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Asynchrony Related to Mechanical Ventilation Exacerbates Diaphragm Dysfunction in a Mouse Model of Duchenne Muscular Dystrophy.

Muscle & nerve·2026
Same author

Structural basis for simvastatin-induced skeletal muscle weakness associated with type 1 ryanodine receptor T4709M mutation.

The Journal of clinical investigation·2025
Same author

L-Type Ca<sub>v</sub>1.3 and HCN Channels Mediate Heart Rate Acceleration by Catecholamines.

Circulation research·2025
Same author

Targeting Calcium Regulation for Heart Failure and Arrhythmia Therapeutics: A Critical Review.

Circulation·2025
Same author

RYR2 Variants in Catecholaminergic Polymorphic Ventricular Tachycardia Patients: Insights From Protein Structure and Clinical Data.

Circulation. Arrhythmia and electrophysiology·2025
Same author

Targeting ryanodine receptors with allopurinol and xanthine derivatives for the treatment of cardiac and musculoskeletal weakness disorders.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Extracellular matrix reprogramming by the YAP/TAZ- TGF-ß2 axis drives immune exclusion in cholangiocarcinoma models.

The Journal of clinical investigation·2026
Same journal

Tumor cell-derived extracellular vesicles foster the immunosuppressive landscape of pancreatic cancer.

The Journal of clinical investigation·2026
Same journal

Julie Zikherman receives the ASCI/Marian W. Ropes, MD, Award.

The Journal of clinical investigation·2026
Same journal

Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models.

The Journal of clinical investigation·2026
Same journal

SGLT2 inhibitors enhance ketogenesis by acting as allosteric activators of the mitochondrial enzyme HMGCS2.

The Journal of clinical investigation·2026
Same journal

MDM2 degraders for Merkel cell carcinoma: round peg in a round hole.

The Journal of clinical investigation·2026
See all related articles

Related Experiment Video

Updated: Jan 7, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K

Rescuing the NIH: the response.

Andrew R Marks

    The Journal of Clinical Investigation
    |May 2, 2006
    PubMed
    Summary
    This summary is machine-generated.

    Feedback on "Rescuing the NIH" reveals a significant gap between National Institutes of Health (NIH) leadership and researchers dependent on extramural funding. This divide impacts scientific progress and resource allocation.

    More Related Videos

    Primary Culture of Mouse Dopaminergic Neurons
    11:58

    Primary Culture of Mouse Dopaminergic Neurons

    Published on: September 8, 2014

    39.1K
    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
    09:54

    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

    Published on: August 10, 2012

    26.4K

    Related Experiment Videos

    Last Updated: Jan 7, 2026

    Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
    09:35

    Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

    Published on: January 20, 2015

    9.2K
    Primary Culture of Mouse Dopaminergic Neurons
    11:58

    Primary Culture of Mouse Dopaminergic Neurons

    Published on: September 8, 2014

    39.1K
    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
    09:54

    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

    Published on: August 10, 2012

    26.4K

    Area of Science:

    • Biomedical Research Funding
    • Science Policy
    • Research Administration

    Background:

    • The editorial
    • Rescuing the NIH before it is too late
    • elicited diverse reader feedback, highlighting a critical disconnect within the National Institutes of Health (NIH).

    Discussion:

    • A substantial divergence of opinions exists between NIH leadership and the extramural research community.
    • This divide pertains to the allocation and management of research funding, impacting scientific endeavors.

    Key Insights:

    • The feedback underscores a chasm in perspectives regarding NIH operations and priorities.
    • Researchers relying on extramural funding feel their needs and challenges are not fully understood by NIH administration.

    Outlook:

    • Addressing this divide is crucial for the future health and effectiveness of the NIH.
    • Bridging the gap requires open dialogue and policy adjustments to better support the extramural research base.