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Digestion of I-PpoI recognition sites in unfavorable sequence contexts achieved by changing the reaction conditions

Christina B McCarthy1, Victor Romanowski

  • 1IBBM, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.

Biochemical Genetics
|May 2, 2006
PubMed
Abstract

No abstract available in PubMed .

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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