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Updated: Aug 9, 2026
10:12
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Published on: September 27, 2016
Divide and conquer: development and cell cycle genes in plant transformation
Renée S Arias1, Sergei A Filichkin, Steven H Strauss
1Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA.
Trends in Biotechnology
|May 3, 2006
Summary
Improving plant transformation and regeneration involves controlling cell cycle genes. Manipulating genes like RBR, VIP1, IPT, and ESR1 enhances stable transformation rates in plants, making genetic engineering more feasible.
Area of Science:
- Plant Biotechnology
- Molecular Biology
- Epigenetics
Background:
- Genetic transformation and regeneration are challenging for most plant species.
- Improving plant transformation efficiency is crucial for crop improvement and research.
Purpose of the Study:
- To explore epigenetic control of gene expression for enhancing plant transformation and regeneration.
- To investigate the role of cell cycle regulation in improving plant transformation competence.
Main Methods:
- Modulating cell cycle and developmental genes through inducible expression or suppression.
- Altering chromatin structure and epigenetic control of gene expression.
- Targeting genes involved in cell cycle transitions (G1-S) and pluripotency.
Main Results:
- Transformation efficiency is higher in cells with nuclei in S and G2 phases.
- Manipulating genes promoting the G1-S transition increased transient and stable transformation.
- Direct cell cycle control (RBR, VIP1) and hormone regulation (IPT, ESR1) improved stable transformation rates.
Conclusions:
- Inducible gene expression systems offer a promising strategy to enhance plant transformation and regeneration.
- Targeting specific cell cycle and epigenetic regulators can significantly improve plant genetic engineering.
- Future research can leverage genomic data and expression systems to identify homologous genes for broader application.
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