Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

From genome to proteome: developing expression clone resources for the human genome.

Gary Temple1, Philippe Lamesch, Stuart Milstein

  • 1Mammalian Gene Collection, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA. gtemple@mail.nih.gov

Human Molecular Genetics
|May 3, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A pH-Tolerant Nickel-Vanadium Phosphonate Framework for Stable Aqueous Supercapacitor Cycling.

ACS nano·2026
Same author

Experimental assessment of AI-based interactome mapping.

Nature communications·2026
Same author

A scalable approach to resolving variants of uncertain significance.

bioRxiv : the preprint server for biology·2026
Same author

Effector-host interactome map links type III secretion systems in healthy gut microbiomes to immune modulation.

Nature microbiology·2026
Same author

Viral transcriptional regulators extensively rewire host pathways through diverse mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

Predicting interaction-specific protein-protein interaction perturbations by missense variants with MutPred-PPI.

bioRxiv : the preprint server for biology·2026

Human cDNA clones are crucial for understanding protein structure and function. This review covers major cDNA collections, their limitations, and proteomics applications like protein-protein interaction analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Complementary DNA (cDNA) clones are essential tools for protein research.
  • The availability of the human genome sequence enables validation of mRNA and protein-coding annotations.
  • Well-characterized human cDNA collections are vital for defining gene and protein structure/function.

Purpose of the Study:

  • To review major human cDNA clone collections.
  • To discuss common limitations of these collections.
  • To highlight proteomics applications, particularly protein-protein interaction (PPI) analysis.

Main Methods:

  • Review of existing literature and databases on human cDNA collections.
  • Categorization of cDNA clones into full-length protein coding sequences (FL-CDS) and full-ORF (F-ORF) expression clones.

Related Experiment Videos

  • Analysis of proteomics applications utilizing these cDNA resources.

    • Main Results:

    • Human cDNA collections primarily consist of FL-CDS and F-ORF clones.
    • FL-CDS clones may lack complete 5' and 3' untranslated regions (UTRs).
    • F-ORF clones contain annotated coding sequences optimized for protein expression.

    Conclusions:

    • Human cDNA clones are indispensable for advancing proteomics and functional genomics.
    • Understanding the characteristics and limitations of cDNA collections is key for effective use.
    • These resources facilitate crucial applications such as the study of protein-protein interactions.