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Updated: Aug 9, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Generation of mice harboring a Sox6 conditional null allele
Bogdan Dumitriu1, Peter Dy, Patrick Smits
1Department of Biomedical Engineering and Orthopaedic Research Center, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Genesis (New York, N.Y. : 2000)
|May 3, 2006
Summary
Researchers developed conditional knockout mice for Sox6, a crucial transcription factor. These new Sox6 conditional null mice enable detailed studies of its functions in specific tissues and postnatal development.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- Sox6 is a transcription factor critical for cell fate and differentiation in various tissues.
- Previous studies of Sox6 knockout mice were limited by early lethality and multiple defects.
Purpose of the Study:
- To generate a conditional knockout mouse model for Sox6 to overcome limitations of previous models.
- To facilitate in-depth investigation of Sox6 roles in specific cell types and postnatal development.
Main Methods:
- Generation of Sox6 conditional null allele (Sox6(fl+)) by flanking the second coding exon with loxP sites.
- Cre-mediated recombination to convert the floxed allele into a null allele (Sox6(fl-)) in somatic and germ cells.
Main Results:
- Sox6(fl+/fl+) mice are phenotypically indistinguishable from wildtype mice.
- Sox6(fl-/fl-) mice exhibit phenotypes similar to Sox6(-/-) mice, confirming successful gene inactivation.
- The conditional system allows for targeted deletion of Sox6 in specific tissues and at specific times.
Conclusions:
- The developed Sox6 conditional null mice are a valuable tool for studying Sox6 function in vivo.
- This model will enable detailed analysis of Sox6's roles in various biological processes, overcoming previous experimental limitations.
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