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Updated: Aug 8, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
Inactivation of maize leaf phosphoenolpyruvate carboxylase by the binding to chloroplast membranes
1Department of Biochemistry, University of California, Riverside, California 92521.
Abstract:
Phosphoenolpyruvate carboxylase (PEPC) purified from maize (Zea mays L.) leaves associates with maize leaf chloroplast membrane in vitro. The binding of PEPC to the membrane results in enzyme inactivation. A protein isolated from a maize leaf chloroplast membrane preparation inactivated PEPC. Treatment with membrane preparation or with partially purified inactivating protein accelerates PEPC inactivation at low temperature (4 degrees C). Interaction of PEPC with chloroplast membrane or inactivating protein may inactivate the enzyme by influencing dissociation of the enzyme active tetramer.
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