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Related Experiment Videos

Plastid DNA in developing maize endosperm : genome structure, methylation, and transcript accumulation patterns.

A J McCullough1, J Kangasjarvi, B G Gengenbach

  • 1Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, University of Minnesota, St. Paul, Minnesota 55108.

Plant Physiology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

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Maize endosperm amyloplasts contain an intact plastid genome. Plastid genes are transcribed during amyloplast development, though at lower levels than in leaves.

Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Amyloplasts are key starch-storing organelles in maize endosperm.
  • Their biogenesis involves plastid development, but the role of the plastid genome is unclear.

Purpose of the Study:

  • To characterize the maize plastid genome in endosperm amyloplasts.
  • To investigate plastid gene transcription during amyloplast biogenesis.

Main Methods:

  • Hybridization of plastid genome clones to endosperm DNA.
  • Analysis of DNA methylation using restriction enzymes.
  • RNA analysis to detect plastid gene transcripts.

Main Results:

  • Endosperm plastid DNA is identical to leaf plastid DNA.

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  • No significant DNA methylation was detected in endosperm amyloplasts.
  • Plastid gene transcripts are present in endosperm, with varying abundance compared to leaves.
  • Some transcripts accumulate as unprocessed or partially processed forms.

    • Conclusions:

    • Maize endosperm cells possess an intact and transcriptionally active plastid genome.
    • Plastid genes can potentially contribute to amyloplast biogenesis.
    • No endosperm-specific transcripts were identified.