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Related Experiment Videos

Accessory cell-derived costimulatory signals regulate T cell proliferation.

K B Urdahl1, M K Jenkins, S D Norton

  • 1Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.

Annals of the New York Academy of Sciences
|December 30, 1991
PubMed
Summary

Anti-CD28 antibody and accessory cells provide similar costimulatory signals for T cell activation, suggesting CD28 interaction with APC ligands delivers this crucial signal for T cell proliferation and IL-2 production.

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Area of Science:

  • Immunology
  • Cellular Biology

Background:

  • T cell activation requires both antigen-specific signals and costimulatory signals.
  • CD28 is a key costimulatory receptor on T cells.

Purpose of the Study:

  • To investigate the similarities between the costimulatory effects of anti-CD28 antibody and accessory cells on T cells.
  • To explore the functional consequences of CD28-mediated costimulation.

Main Methods:

  • Stimulation of T cells with immobilized anti-CD3 antibody or antigen-presenting cells (APCs).
  • Assessment of T cell proliferation and interleukin-2 (IL-2) production.
  • Evaluation of T cell responses in the presence of cyclosporin A and phorbol ester.

Main Results:

  • Both anti-CD28 antibody and accessory cells promoted T cell proliferation and IL-2 production when primary T cell activation signals were suboptimal.

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  • Both stimuli induced cyclosporin A-resistant T cell proliferation in conjunction with phorbol ester.
  • The costimulatory effects of anti-CD28 antibody closely mimicked those of accessory cells.

    • Conclusions:

    • Anti-CD28 antibody binding to T cells replicates the costimulatory signals normally provided by the interaction of CD28 with ligands on accessory cells.
    • This suggests a conserved mechanism for CD28-mediated costimulation in T cell activation.