Related Experiment Video
Updated: Jun 25, 2026
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 28, 2014
Isolation and identification of indole-3-ethanol (tryptophol) from cucumber seedlings
1Department of Biological Sciences, University of California, Santa Barbara, California 93106.
Abstract:
Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics.
