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delta-Aminolevulinic Acid Transaminase in Chlorella vulgaris
Plant Physiology
|September 1, 1968
Abstract:
An enzyme catalyzing the formation of delta-aminolevulinic acid by transamination of gamma,delta-dioxovaleric acid with l-alpha-alanine, l-glutamic acid, or l-phenylalanine has been detected in extracts of Chlorella vulgaris. The activity of this enzyme does not appear to parallel changes in chlorophyll content in a Chlorella mutant which requires light for chlorophyll production. The role of this enzyme in delta-aminolevulinic acid metabolism in plants is not clearly understood.
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Aminolysis is a nucleophilic acyl substitution reaction, where ammonia or amines act as nucleophiles to give the substitution product. Acid halides react with ammonia, primary amines, and secondary amines to yield primary, secondary, and tertiary amides, respectively.
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In the first step of the aminolysis mechanism, the amine attacks the carbonyl carbon of the acyl chloride to form a tetrahedral intermediate. In the second step, the carbonyl group is re-formed with the elimination of a chloride...

