Related Experiment Video
Updated: Aug 4, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
Published on: March 5, 2017
Ribonuclease Activity in Normal, opaque-2, and floury-2 Maize Endosperm during Development
1Department of Botany and Plant Pathology and Department of Biochemistry, Purdue University, Lafayette, Indiana 47907.
Abstract:
The elevated ribonuclease activity produced in the endosperm of a maize (Zea mays L.) inbred, W64A, by homozygous opaque-2, results from a more than doubled rate of ribonuclease accumulation occurring prior to 16 days post-pollination; after 16 days the rates in opaque-2 and normal are the same, suggesting that opaque-2 is no longer active. The pattern of ribonuclease increase in the opaque-2 dosage series indicates that opaque-2 is not fully recessive. Ribonuclease accumulation is not affected by floury-2 in a second inbred, B14. The results are discussed with reference to other proteins, notably zein, the net synthesis of which is affected by opaque-2.
Related Concept Videos
Ribozymes
Ribozymes can be...
Protein Folding Quality Check in the RER

