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Related Experiment Videos

Phytochrome-controlled Leaf Unrolling and Protein Synthesis.

B G Kang1

  • 1Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.

Plant Physiology
|March 1, 1971
PubMed
Summary

Red light triggers wheat seedling leaf unrolling by increasing soluble protein synthesis, a process influenced by plant hormones and inhibited by specific drugs. This suggests a critical role for newly synthesized proteins in the photomorphogenic response.

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Plant & cell physiology·2001

Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Photomorphogenesis

Background:

  • Etiolated seedlings exhibit photomorphogenic responses to light.
  • Leaf unrolling in wheat (Triticum aestivum L.) is a light-dependent process.
  • Protein synthesis is a fundamental cellular mechanism.

Purpose of the Study:

  • To investigate the role of protein synthesis in red light-induced leaf unrolling in wheat.
  • To identify the specific protein fractions involved in this photomorphogenic response.
  • To explore the influence of plant hormones and inhibitors on light-mediated leaf unrolling.

Main Methods:

  • Differential centrifugation to isolate cellular fractions.
  • Incorporation of (14)C-leucine to measure protein synthesis.

Related Experiment Videos

  • Treatment with inhibitors (cycloheximide, chloramphenicol) and plant hormones (indoleacetic acid, abscisic acid, kinetin).
  • Studies on a barley (Hordeum vulgare L.) albino mutant.

    • Main Results:

    • Red light increased (14)C-leucine incorporation into the supernatant protein fraction, correlating with leaf unrolling.
    • Inhibitors blocked both leaf unrolling and supernatant protein synthesis.
    • A barley mutant lacking ribulose diphosphate carboxylase showed red light-promoted supernatant protein synthesis and leaf unrolling.
    • Plant hormones modulated protein synthesis and leaf unrolling, with kinetin inducing unrolling in darkness.
    • Red light promoted protein synthesis even when unrolling was osmotically inhibited.

    Conclusions:

    • Red light-induced leaf unrolling in wheat seedlings is dependent on the synthesis of soluble proteins.
    • The supernatant protein fraction plays a key role in mediating this photomorphogenic response.
    • Plant hormones interact with light signaling pathways to regulate leaf development.