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Related Experiment Videos

Invertase Activity in Normal and Mutant Maize Endosperms during Development.

T A Jaynes1, O E Nelson

  • 1Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana 47907.

Plant Physiology
|May 1, 1971
PubMed
Summary

Maize endosperm contains three invertase enzymes, including two soluble forms (invertase I and II) and one bound form. Invertase I activity peaks early, but shows a later increase in specific maize mutants.

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Area of Science:

  • Plant Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Invertases are crucial enzymes in plant carbohydrate metabolism.
  • Understanding invertase activity in developing maize (Zea mays L.) endosperm is key to deciphering sugar partitioning and utilization.

Purpose of the Study:

  • To characterize the different forms of invertase present in developing maize endosperm.
  • To investigate the temporal expression and kinetic properties of these invertase isoforms.

Main Methods:

  • Enzyme extraction and separation using diethylaminoethyl-cellulose and Sephadex chromatography.
  • Kinetic analysis to distinguish between soluble invertase isoforms.
  • Assaying invertase activity at various stages of endosperm development in wild-type and mutant maize lines.

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Main Results:

  • Three distinct invertase forms were identified: bound invertase, soluble invertase I, and soluble invertase II.
  • Soluble invertase I exhibited maximal activity at 12 days post-anthesis and was present from 10 to 28 days.
  • Soluble invertase II was detected in very young endosperm (6 days) and in the embryo during germination. Bound invertase activity remained relatively constant throughout development.
  • Mutant lines (shrunken-1, shrunken-2) showed a secondary increase in invertase I activity later in development.

Conclusions:

  • Maize endosperm possesses multiple, differentially regulated invertase enzymes.
  • The distinct temporal patterns of invertase I and II suggest specialized roles during endosperm development and germination.
  • Abnormal metabolism in maize mutants may influence invertase activity patterns.