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Updated: Aug 8, 2026

Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
Enhanced deoxyribonucleic Acid polymerase activity of chromatin from soybean hypocotyls treated with
H R Leffler1, T J O'brien, D V Glover
1Departments of Agronomy and Horticulture, Purdue University, Lafayette, Indiana 47907.
Abstract:
Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydrolysis and ribonuclease, but was sensitive to acid hydrolysis and deoxyribonuclease. Chromatin-DNA polymerase required Mg(2+) and all four deoxyribonucleoside triphosphates for maximal activity. Inorganic pyrophosphate and actinomycin D inhibited the polymerase activity, but 2, 4-dichlorophenoxyacetic acid had no effect in vitro. Chromatin from plants previously treated with 2, 4-dichlorophenoxyacetic acid supported a greater level of DNA synthesis than did chromatin from untreated plants.
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