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Related Experiment Videos

Development of chlorophyll and hill activity.

K D Nadler1, H A Herron, S Granick

  • 1Rockefeller University, New York, New York 10021.

Plant Physiology
|March 1, 1972
PubMed
Summary

The development of oxygen evolution in barley leaves depends on protein synthesis in both cytoplasm and plastids, not chlorophyll synthesis. Prolonged light exposure is crucial for photosynthesis to begin.

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Area of Science:

  • Plant Physiology
  • Photosynthesis Research
  • Molecular Biology

Background:

  • Greening of etiolated leaves involves complex biochemical processes.
  • Oxygen evolution is a key indicator of photosystem II activity.
  • The interplay between chlorophyll synthesis and protein synthesis in photosynthesis is not fully understood.

Purpose of the Study:

  • To investigate the factors limiting the onset of oxygen evolution during the greening of barley leaves.
  • To determine whether chlorophyll synthesis or protein synthesis regulates the development of photosystem II activity.
  • To elucidate the roles of cytoplasmic and plastid protein synthesis in photosynthetic development.

Main Methods:

  • Utilized a sensitive luminometer to directly measure oxygen evolution rates in barley leaf segments.
  • Infiltrated leaf segments with p-benzoquinone to facilitate oxygen evolution measurements.
  • Administered delta-aminolevulinic acid to induce chlorophyll synthesis and chloramphenicol/cycloheximide to inhibit protein synthesis.

Main Results:

  • Oxygen evolution was significantly delayed until the end of the chlorophyll synthesis lag phase.
  • Increasing chlorophyll levels with delta-aminolevulinic acid did not accelerate photosynthesis.
  • Chloramphenicol and cycloheximide strongly inhibited oxygen evolution while only slightly affecting chlorophyll synthesis.
  • Oxygen evolution capacity remained low in darkness, irrespective of chlorophyll levels.

Conclusions:

  • The development of photosystem II and oxygen evolution is primarily limited by protein synthesis (cytoplasmic and plastid), not chlorophyll synthesis.
  • Sufficient protein synthesis and prolonged illumination are necessary prerequisites for functional photosystem II and oxygen evolution.

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