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Updated: Aug 8, 2026

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
The levels of soluble nucleotides in wheat aleurone tissue
G G Collins1, C F Jenner, L G Paleg
1Department of Plant Physiology, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, South Australia.
Abstract:
The content of soluble nucleotides in aleurone layers isolated from mature wheat (Triticum aestivum var. Olympic) grain was investigated. The most abundant nucleotides were adenosine triphosphate, uridine triphosphate, and uridine diphosphoglucose. Smaller amounts of guanosine triphosphate, cytidine triphosphate, adenosine diphosphate, and nicotinamide adenine dinucleotide were also identified. The levels of some of these nucleotides were increased after incubation of the tissue under certain conditions.Nucleotide levels were measured at intervals during incubation of aleurone layers in water. The changes observed are discussed in relation to a response by the tissue to wounding.
