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Related Experiment Videos

Induction and development of increased ion absorption in corn root tissue.

R T Leonard1, J B Hanson

  • 1Department of Botany, University of Illinois, Urbana, Illinois 61801.

Plant Physiology
|March 1, 1972
PubMed
Summary

Washing corn roots enhances nutrient ion and solute uptake by activating existing transport mechanisms. This process requires aerobic metabolism and protein synthesis, but not cell proliferation.

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Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Root nutrient uptake is crucial for plant growth.
  • Understanding the regulation of ion transport is essential for agricultural productivity.

Purpose of the Study:

  • To investigate the effects of washing on nutrient ion and solute accumulation in corn roots.
  • To elucidate the underlying mechanisms of this response.

Main Methods:

  • Washing intact or excised corn roots in aerated solutions.
  • Measuring nutrient ion and solute uptake rates.
  • Utilizing inhibitors of RNA and protein synthesis.
  • Performing kinetic analyses and electron microscopy.

Main Results:

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  • Washing doubled the rate of nutrient ion and solute accumulation.
  • Phosphate absorption increased significantly after a lag period.
  • The response depended on aerobic metabolism and protein synthesis, but not increased respiration.
  • No changes in membrane structure or kinetic parameters were observed.

Conclusions:

  • Washing activates existing membrane transport mechanisms in corn roots.
  • This activation involves protein synthesis and aerobic metabolism.
  • The findings offer insights into regulating nutrient uptake in plants.