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Comparative immunochemistry of phytochrome.

L H Pratt1

  • 1Department of General Biology, Vanderbilt University, Nashville, Tennessee 37235.

Plant Physiology
|January 1, 1973
PubMed
Summary
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Phytochrome, a plant photoreceptor, shows high similarity across oat, rye, barley, and pea species, with minor antigenic differences noted, particularly with pea phytochrome. Both large and small phytochrome forms from oats are antigenically similar.

Area of Science:

  • Plant molecular biology
  • Photoreceptor research
  • Protein biochemistry

Background:

  • Phytochrome is a crucial plant photoreceptor regulating various developmental processes.
  • Understanding phytochrome structure and evolution across species is vital for plant science.
  • Previous research has identified different molecular weight forms of phytochrome.

Purpose of the Study:

  • To purify and characterize high molecular weight phytochrome from multiple plant species.
  • To compare the antigenic properties of phytochrome from oats, rye, barley, and peas.
  • To investigate the relationship between large and small molecular weight forms of phytochrome in oats.

Main Methods:

  • Multi-step chromatographic purification (brushite, DEAE-cellulose, Bio-Gel P-300).

Related Experiment Videos

  • Production and characterization of specific antisera against low molecular weight phytochrome.
  • Immunological assays: double diffusion, micro complement fixation, and immunoelectrophoresis.

    • Main Results:

    • High molecular weight phytochrome preparations (approx. 440,000 MW) were obtained from oats, rye, barley, and peas.
    • Antigenic identity was observed among oat, rye, and barley phytochrome, with notable differences for pea phytochrome.
    • Micro complement fixation showed complete identity between oat varieties, reduced activity with rye and barley, and no activity with pea phytochrome.
    • Large and small phytochrome forms from oats were antigenically similar, despite differences in electrophoretic mobility.

    Conclusions:

    • Phytochrome exhibits significant antigenic conservation across diverse plant species, with variations suggesting evolutionary divergence.
    • The purification and characterization of phytochrome from multiple sources provide a basis for further comparative studies.
    • The study confirms the antigenic similarity between different molecular weight forms of phytochrome within the same species.