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Related Experiment Video

Updated: Jul 17, 2026

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
05:38

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Published on: February 8, 2019

Hexokinase from maize endosperm and scutellum.

E L Cox1, D B Dickinson

  • 1Department of Horticulture, University of Illinois, Urbana, Illinois 61801.

Plant Physiology
|May 1, 1973
PubMed
Summary

Maize seed hexokinase activity differs between endosperm and scutellum tissues. The scutellum enzyme shows higher affinity for fructose, suggesting potential regulatory roles and the presence of glucokinase.

Area of Science:

  • Plant Biochemistry
  • Enzymology
  • Maize Seed Physiology

Background:

  • Hexokinase (EC 2.7.1.1) plays a crucial role in carbohydrate metabolism in plants.
  • Understanding hexokinase kinetics in different maize seed tissues is vital for comprehending energy utilization during development and germination.

Purpose of the Study:

  • To isolate and characterize hexokinase from developing and germinating maize (Zea mays) seeds.
  • To investigate kinetic differences of hexokinase in endosperm and scutellum tissues using fructose and glucose as substrates.
  • To explore potential regulatory mechanisms and the presence of other hexose kinases.

Main Methods:

  • Isolation of hexokinase enzyme from maize endosperm and scutellum.
  • Enzyme kinetic studies using varying concentrations of fructose and glucose as substrates.

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  • Determination of Michaelis constant (Km) values for different tissue-substrate combinations.

    • Main Results:

    • Scutellum hexokinase exhibited approximately one-third the Michaelis constant for fructose compared to endosperm hexokinase (0.05 vs. 0.15 mM).
    • No significant developmental differences in Michaelis constant values were observed for fructose.
    • Michaelis constant values for glucose ranged from 0.1 to 0.2 mM across tissues and developmental stages.
    • A secondary, higher Michaelis constant (3.4 mM) for glucose was identified in germinating scutellum, indicating low-affinity binding.

    Conclusions:

    • Maize scutellum hexokinase demonstrates higher affinity for fructose than endosperm hexokinase.
    • The presence of a low-affinity glucose-binding site in germinating scutellum hexokinase suggests potential regulatory functions.
    • These findings may indicate the co-existence of hexokinase and glucokinase activities in maize scutellum.