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Updated: Jul 25, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Translocation of iron from soybean cotyledons
1Agricultural Environmental Quality Institute, Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705.
Abstract:
Soybean seeds, Glycine max L. Merrill, were produced by plants treated from anthesis to seed maturity with (59)Fe supplied as ferric ethylenediaminedi (o-hydroxyphenylacetate). Seed coats accounted for 7.4% of dry seed weight and had Fe concentrations 5 times greater than the embryos. After germinating 2 days, cotyledons contained 69.6% and radicles 5.0% of original seed Fe. Fractions of seed Fe unavailable to seedlings were: 19.8% in seed coats, 1.7% in germination paper, 0.1% in the water under germinating seeds, and 3.8% unaccounted for. Every 3 days seedlings received nutrient solution without Fe or with 10 mum ferric ethylenediaminedi (o-hydroxyphenylacetate) and developed as deficient Fe or normal Fe plants. The deficient Fe cotyledons on day 18 retained 13% of the labeled Fe originally present. Cotyledons of normal Fe plants retained 50 to 70% of their original Fe. Moreover, cotyledons of the normal Fe plants accumulated externally supplied Fe and finally contained twice the quantity of Fe originally present. Stem exudate collected above cotyledons of deficient Fe plants contained 5.3 mum(59)Fe. Electrophoresis of exudate showed that most of the (59)Fe migrated anodically as a single band and was in the position of ferric citrate.
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